Composite
Part:BBa_K777129
Designed by: Team Goettingen Group: iGEM12_Goettingen (2012-09-20)
Constitutive J23109 promoter
This part is a twin of an Anderson promoter in K777133, a pSB1C3 derived backbone.
Usage and Biology
This construct can be useful in several ways, just like the similar partJ61002. However, if you plan to send coding or composite parts to the registry, they are already in the correct backbone here, pSB1C3.
- You may use this plasmid to insert a promoter of your choice between the XbaI/EcoRI and SpeI sites. This will provide you with a downstream mRFP as a reporter. Look up the specifications for restriction sites here.
- Furthermore, you can clone a gene of interest downstream of the constitutive Anderson promoters to express it at a desired rate. For this, the SpeI and PstI sites can be used. Look up the specifications for restriction sites here.
- It can provide a nice selection system for inserting your basic parts into the pSB1C3 vector via the EcoRI/XbaI and PstI site. If you use a construct containing a strong promoter e.g. K777125, colonies containing a self-ligated plasmid after transformation will turn red and you can select for the non-red colonies.
- Here is the complete sequence as an ApE file
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 8
Illegal NheI site found at 31 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/prokaryote/ecoli
//promoter/anderson
//promoter/anderson
positive_regulators | Anderson promoter |