Coding

Part:BBa_K945006

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-27)
Revision as of 08:45, 1 October 2012 by DanielV (Talk | contribs)

Pichia pastoris optimized His-CrtE-PTS1

This sequence codes for CrtE (GGPP synthase), codon optimized for expression in Pichia pastoris<i/> and engineered to include a 6xHis-tag in its N-terminus and the peroxisomal targeting signal type 1 (PTS1) in its C-terminus. It also includes a yeast Kozak consensus sequence and the AOX1 transcription terminator sequence for its use in expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1346
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1318
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1346
    Illegal AgeI site found at 1037
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1301

[edit]
Categories
Parameters
None