Reporter

Part:BBa_K945004

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-26)
Revision as of 07:15, 1 October 2012 by DanielV (Talk | contribs)

Pichia pastoris optimized His-GFP

This sequence codes for a wild-type green fluorescent protein (GFP) which has been codon optimized for Pichia pastoris and engineered to include a 6xHis-tag in its N-terminus. It also includes the AOX1 transcription terminator sequence for its use in expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 25
    Illegal XbaI site found at 1315
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 25
    Illegal BamHI site found at 1286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 25
    Illegal XbaI site found at 1315
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 25
    Illegal XbaI site found at 1315
    Illegal AgeI site found at 1005
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 877
    Illegal BsaI.rc site found at 1269


[edit]
Categories
Parameters
None