Tag

Part:BBa_K880004

Designed by: Josh Atkinson, Mike Ferguson, and Ben Parker   Group: iGEM12_Michigan   (2012-09-29)
Revision as of 21:27, 30 September 2012 by Cbate (Talk | contribs)

LVA Tag in RFC25

The amino acid sequence AANDENYALVA can be fused onto proteins in order to rapidly reduce their half life in the cytoplasm. Responsiveness for genetic circuits can be improved by c-terminal fusions of this short, handy sequence.

This part possesses an RFC25 prefix to facilitate in-frame translation.

Reference: Andersen et al. Appl Environ Microbiol. 1998 June; 64(6): 2240–2246.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None