Generator
Part:BBa_K741003
Designed by: Wuyang Chen Group: iGEM12_USTC-China (2012-07-24)
pRM-RBS-GFP-T
Express GFP only if protein CI activated the promoter pRM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 752
Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.
Data
| pRM-RBS-GFP-T | |||
| OD | |||
| t/min | Parallel | Experimental group | M9 medium |
| 0 | 1 | 0.0593 | 0.045 |
| 2 | 0.0642 | ||
| 3 | 0.0678 | ||
| 45 | 1 | 0.0605 | 0.0436 |
| 2 | 0.0734 | ||
| 3 | 0.062 | ||
| 90 | 1 | 0.0648 | 0.0435 |
| 2 | 0.0735 | ||
| 3 | 0.0702 | ||
| 135 | 1 | 0.0811 | 0.0447 |
| 2 | 0.0716 | ||
| 3 | 0.0677 | ||
| 180 | 1 | 0.0723 | 0.0446 |
| 2 | 0.0828 | ||
| 3 | 0.0789 | ||
| pRM-RBS-GFP-T | |||
| fluorescence intensity | |||
| t/min | Parallel | Experimental group | M9 medium |
| 0 | 1 | 19.55 | 11.801 |
| 2 | 19.571 | ||
| 3 | 19.352 | ||
| 45 | 1 | 21.548 | 13.386 |
| 2 | 20.505 | ||
| 3 | 19.107 | ||
| 90 | 1 | 24.441 | 11.541 |
| 2 | 23.777 | ||
| 3 | 24.503 | ||
| 135 | 1 | 28.938 | 11.894 |
| 2 | 27.884 | ||
| 3 | 27.81 | ||
| 180 | 1 | 41.376 | 11.066 |
| 2 | 39.573 | ||
| 3 | 39.795 | ||
[edit]
Categories
Parameters
| None |