Part:BBa_K845005:Design
TapZ engineered membrane receptor
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Tap and EnvZ were extracted from BW25133 E. Coli.
We used an overlapping PCR to assemble the sequences.
Our safety sheet: https://static.igem.org/mediawiki/2012/5/53/TapZ.pdf
Source
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References
Manson MD, Blank V, Brade G, Higgins CF.Peptide chemotaxis in E. coli involves the Tap signal transducer and the dipeptide permease. Nature. 1986 May 15-21;321(6067):253-6.
Maeda S, Takayanagi K, Nishimura Y, Maruyama T, Sato K, Mizuno T. Activation of the osmoregulated ompC gene by the OmpR protein in Escherichia coli: a study involving synthetic OmpR-binding sequences. J Biochem. 1991 Sep;110(3):324-7.
Weerasuriya S, Schneider BM, Manson MD. Chimeric chemoreceptors in Escherichia coli: signaling properties of Tar-Tap and Tap-Tar hybrids. J Bacteriol. 1998 Feb;180(4):914-20.
Cai SJ, Inouye M. EnvZ-OmpR interaction and osmoregulation in Escherichia coli. J Biol Chem. 2002 Jul 5;277(27):24155-61. Epub 2002 Apr 24.
Baumgartner JW, Kim C, Brissette RE, Inouye M, Park C, Hazelbauer GL. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ. J Bacteriol. 1994 Feb;176(4):1157-63.