Reporter
sfGFP

Part:BBa_K812331

Designed by: Tristan Cerisy   Group: iGEM12_Evry   (2012-09-20)
Revision as of 16:49, 30 September 2012 by TriCer (Talk | contribs)

sfGFP in pCS2+ with HSP70 promoter



*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812031. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000 (CMV promoter), BBa_K812300 (HSP70 promoter), BBa_K812200 (elastase promoter).***

Super folded Green fluorescent protein (sfGFP) fused with a kozak sequence to enhance its expression in eukaryotic cells. This part is in pCS2+, the plasmid backbone is BBa_K812300
This part was created with the plasmid backbone BBa_K812300: J04450 was replaced by sfGFP (BBa_K812031) and the CMV promoter was replaced by the Hsp70 (Heat Shock Promoter) promoter promoter with SalI and HindIII restriction sites from BBa_K812300.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19


[edit]
Categories
Parameters
None