Part:BBa_J45001

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter

From snapdragon (A. Majus)

(GenBank Accession No. AF515284)
It is a homodimer with subunit molecular mass of about 45 kDA
On the pET-28a expression vector there is a convenient NdeI site at the initiating ATG codon as well as a BamHI site downstream of the stop codon. Natalia Dudareva is sending the pET-28a vector

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 577
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 901
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 421
Illegal NgoMIV site found at 425
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 471
Illegal SapI.rc site found at 75