Part:BBa_J45001

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter

From snapdragon (A. Majus)

Usage and Biology

1. (GenBank Accession No. AF515284)
2. It is a homodimer with subunit molecular mass of about 45 kDA
3. On the pET-28a expression vector there is a convenient NdeI site at the initiating ATG codon as well as a BamHI site downstream of the stop codon. Natalia Dudareva is sending the pET-28a vector

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 577
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 901
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 421
  Illegal NgoMIV site found at 425
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 471
  Illegal SapI.rc site found at 75