Measurement

Part:BBa_K741004

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)
Revision as of 08:12, 30 September 2012 by Dunkle (Talk | contribs) (zs)

plac-RBS-cI-T-pRM-RBS-GFP-T

Protein CI is expressed to activated the promoter pRM. Thus different concentration of CI lead to different strength of green fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1898

USTC2012Result3 副本.png

Figure 3 shows similar effects of glucose and IPTG on plac-cI-pRM-gfp. pRM is activated by the protein cI. By adding IPTG, plac is activated, which leads to the production of the protein cI. With the existence of cI, pRM-GFP starts to work. On the contrast, glucose represses the expression of plac, which reduces the protein cI. Therefore, pRM expresses little and the unit fluorescence intensity of experimental groups with glucose added is the lowest.

USTC2012Result4 副本.png

Figure 4 shows the difference of the unit fluorescence intensity between plac-gfp and plac-cI-pRM-gfp. Generally, the fluorescence intensity of plac-gfp is higher than that of plac-cI-pRM-gfp, because the transcription efficiency of pRM is quite low, which reduces the expression of gfp in comparison with plac-gfp.

[edit]
Categories
Parameters
None