DNA

Part:BBa_K864050:Design

Designed by: Erik Lundin   Group: iGEM12_Uppsala   (2012-09-25)
Revision as of 23:51, 29 September 2012 by Erigu863 (Talk | contribs) (→‎Source)

pSC101 low copy origin of replication


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2130
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This ori was created by cloning pSC101ts from pSIM5 and making a V56A mutation in repA.


Source

The pSC101ts origin was cloned from the pSIM5 plasmid from Datta et al, see reference below.

References

[http://dx.doi.org/10.1016/j.gene.2006.04.018] Datta, S., Costantino, N. & Court, D.L. A set of recombineering plasmids for gram-negative bacteria. Gene 379, 109–115 (2006).