Reporter

Part:BBa_K779302

Designed by: Giulio Alighieri   Group: iGEM12_MIT   (2012-09-29)
Revision as of 21:01, 29 September 2012 by Rlearsch (Talk | contribs)

eYFP-4xFF1 MammoBlock

This part is an engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces yellow. Additionally, this protein has four binding sites for the FF1 micro RNA (miRNA). In the presence of FF1 miRNA, translation of this protein is markedly attenuated.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 850
    Illegal PstI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 850
    Illegal PstI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 850
    Illegal PstI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 850
    Illegal PstI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aeYFP-4xFF1 MammoBlock