Regulatory

Part:BBa_K779405

Designed by: Divya Israni   Group: iGEM12_MIT   (2012-09-29)
Revision as of 20:46, 29 September 2012 by Disrani (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

U6-TetO-DecoyFF4-3p-4ntin MammoBlock

The U6-TetO-DecoyFF4-3p-4ntin part encodes conditional expression of the short RNA sequence DecoyFF4-3p-4ntin. The mammalian RNA Pol III U6 promoter incorporates tetracycline-responsive elements that allow for tightly regulated RNA expression under specific conditions. In the presence of the Tet repressor (TetR), two Tet operator (TetO) sites become bound by the TetR and prevent RNA Pol III from binding, subsequently inhibiting transcription of the downstream sequence. In the absence of TetR, RNA transcription proceeds normally. The short DecoyFF4-3p-4ntin RNA sequence can be used as an RNA interference mechanism through its binding to a complementary region present in the micro RNA sequence FF4 (miR-FF4), thus inhibiting the RNA interference activity of the miR-FF4. Ultimately, the gene that miR-FF4 generally silences is allowed to be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aU6-TetO-DecoyFF4-3p-4ntin MammoBlock