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Part:BBa_K779500:Design

Designed by: Kristjan Eerik Kaseniit   Group: iGEM12_MIT   (2012-09-29)
Revision as of 20:22, 29 September 2012 by Eerik (Talk | contribs) (→‎Design Notes)

Long reporter top strand with bulge MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

4-nucleotide bulge added to Part:BBa_K779501. Bulge placed 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by Iba et al. (2011).

See more details at the MIT iGEM wiki: http://2012.igem.org/Team:MIT

Source

De novo design.

References