Reporter

Part:BBa_K779311

Designed by: Katie Bodner   Group: iGEM12_MIT   (2012-09-29)
Revision as of 20:11, 29 September 2012 by Felixsun (Talk | contribs)

HH-mKate - Hammerhead attached to front of mKate MammoBlock

Along with Part:BBa_K779312, Part:BBa_K779313, and Part:BBa_K779314, this part introduces in vivo hammerhead ribozymes to iGEM and the registry. Hammerhead ribozymes are self-cutting strands of catalytic RNA. After a hammerhead ribozyme is transcribed in a cell, it folds into a secondary structure, which causes self-cleavage. Because of their RNA-cleaving function, hammerheads may be used to regulate protein expression and to make other RNA parts. For more information on hammerheads in general, see http://en.wikipedia.org/wiki/Hammerhead_ribozyme .

These four parts consist of a hammerhead ribozyme attached to an mKate florescent protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHH-mKate - Hammerhead attached to front of mKate MammoBlock