Device

Part:BBa_K771005

Designed by: Guo Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-25)
Revision as of 10:34, 29 September 2012 by Albertwyq (Talk | contribs) (→‎Membrane Rudder System)

Membrane Anchor 5:ssDsbA-VVD-LGT-GBD Ligand

Membrane anchor 5, which consists of interacting light sensor VVD ,PDZ ligand and membrane protein Lgt. Membrane Anchor 5 is proved to constitutively dimerize with Membrane Anchor 4 [BBa_K771004]. and could aggregate with Membrane Anchor 6 [BBa_K771006] only when blue light is present.

Membrane Rudder System

12SJTU-MA1-MA6.png

Demonstration of the whole Membrane Rudder device.

light sensor

VVD protein is a blue light induced dimer ([BBa_K771109])

Related Biobrick

<br\>Membrabe Anchor 1([BBa_K771001]) <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 6([BBa_K771006])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 494
    Illegal AgeI site found at 1339
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 960


[edit]
Categories
Parameters
n/aMembrane Anchor 5:ssDsbA-VVD-LGT-GBD Ligand