Part:BBa_K771006

Membrane Anchor 6:ssDsbA-VVD-LGT

Membrane anchor 6, which consists of interacting light sensor VVD and membrane protein Lgt. Membrane Anchor 6 is expected to aggregate with Membrane Anchor 5 only when blue light is present[BBa_K771005].

Membrane Rudder System

Demonstration of the whole Membrane Rudder device.

light sensor

Photoreceptor of Neurospora crassa and can form dimer in the presence of blue light and disassociate as light is off. Mutant (C71V and N56K) is harder to dimerize in the dark and easier to disassociate under blue light compared with wildtype VVD

Dark state VVD dimers, from PDB ID 2PD7

Light is an intriguing signal to regulate E.coli activity because it is easy to obtain, highly tunable and nontoxic. A metabolite-coupled light-switchable system could be quite fascinating.

Vivid(VVD) protein, a photoreceptor of Neurospora crassa can form dimer in the presence of blue light and disassociate as light is off. Besises, VVD protein belongs to the Per-Arnt-Sim(PAS) protein superfamily.

Compared with wildtype VVD, VVD mutant (C71V and N56K)(this part), is harder to dimerize in the dark and easier to dimerize under blue light . So this mutant is ideal as light sensor in Membrane Rudder device. The VVD construction connected to original Membrane Anchor is Part:BBa_K771005 and Part:BBa_K771006, for Membrane Anchor 5 and 6.

Whether blue light is present or not could determine which enzymes be assembled to make their final product dominate.

Related Biobrick

Membrabe Anchor 1([BBa_K771001]) <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005])

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 494
  Illegal AgeI site found at 1339
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 960