Part:BBa_K771001
Membrane Anchor 1 :ssDsbA-Lgt-PP7
Membrane anchor 1:RNA aptamer binding protein PP7 BBa_K771111fused with membrane protein
LgtBBa_K771102.
Overview: Membrane Scaffold System
Demonstration of the whole Membrane Scaffold device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate
with constitutive cluster when certain signal is present.
Backgroud
Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway (in this case involving enzyme 1, 2, 3, 4 and 5) is activated or
not. RNA molecule D0 BBa_K771009with PP7 and MS2 aptamer domain acts as the signal.
Design
RNA aptamer binding protein PP7 BBa_K771111fused
with membrane protein LgtBBa_K771102. When RNA molecule D0 is present, Membrane Anchor 1 is
expected to dimerize with Membrane Anchor 2BBa_K771002 through MS2
BBa_K771112 and PP7[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111
BBa_K771111] domain
Related Biobrick
<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])
Reference
Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1129
Illegal BglII site found at 1385 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 883
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 504
n/a | Membrane Anchor 1 :ssDsbA-Lgt-PP7 |