Coding

Part:BBa_K771104

Designed by: Guo Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-24)
Revision as of 03:31, 29 September 2012 by Jucember (Talk | contribs) (→‎Usage and Biology)

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ssDsbA-PDZligand

components of interacting proteins

Usage and Biology

12SJTU 2PDZ.gif

The PDZ ligand along with its domain(Part:BBa_K771103) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the PDZ ligand is located in Membrane Anchor 3, while the PDZ domain on Membrane Anchor 4. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsbA, to help the Membrane Anchor localize to the membrane correctly.

Related Parts

Part:BBa_K771103, PDZ domain

Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand

Part:BBa_K771004, Membrane Anchor 4, containing PDZ domaim

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/assDsbA-PDZligand