Coding

Part:BBa_K729003

Designed by: Bouran Sohrabi   Group: iGEM12_UCL_London   (2012-06-30)
Revision as of 19:39, 28 September 2012 by Boo (Talk | contribs) (→‎Variations)

csgBAEFG operon

Stand alone csgBAEFG operon for curli synthesis. Adapted from Lyon 2011.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1683
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterisation

Congo red binding assays were preformed to determine the presence of curli fibers.

Variations

Two additional variations on the original biobrick were designed. These include a T7 and constitutive promoter.

References

[edit]
Categories
Parameters
None