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Cleavage of selected biobrick

Our proposed system can be utilized as a new safety approach for synthetic biology, especially for iGEMers. As we know, one of the most concerned safety issues in engineering cells is that the leakage of bacteria with transformed plasmid that may cause environmental pollution. As it was proved that CRISPR system can target on plasmid DNA sequence, our system may also function as a tool for biobrick cleavage. Specifically, this tool can be used for targeting and cleaving the antibiotic resistance genes present on the biobricks or the standard site of the biobrick such as prefix, suffix, in order to prevent horizontal gene transfer caused by DNA fragments released from apoptotic pathways. Our system has the potential to be applied as a tool to provide a higher level of the safety control of engineered microorganism machine starting from DNA level.