User contributions
- 10:16, 30 September 2012 (diff | hist) . . (-1) . . Part:BBa K786030 (current)
- 15:15, 28 September 2012 (diff | hist) . . (+3) . . Part:BBa K786031:Experience (→Introduction: New Safety Approach) (current)
- 15:15, 28 September 2012 (diff | hist) . . (-7) . . Part:BBa K786031:Experience (→Introduction: New Safety Approach)
- 15:15, 28 September 2012 (diff | hist) . . (-982) . . Part:BBa K786031:Experience (→Introduction: New Safety Approach)
- 15:12, 28 September 2012 (diff | hist) . . (+35) . . Part:BBa K786031:Experience (→Introduction: New Safety Approach)
- 15:11, 28 September 2012 (diff | hist) . . (+1,564) . . Part:BBa K786031:Experience
- 15:09, 28 September 2012 (diff | hist) . . (-156) . . Part:BBa K786031:Experience
- 15:09, 28 September 2012 (diff | hist) . . (+144) . . Part:BBa K786031 (current)
- 15:06, 28 September 2012 (diff | hist) . . (-1) . . Part:BBa K786031 (→Introduction: New Safety Approach)
- 15:06, 28 September 2012 (diff | hist) . . (+33) . . Part:BBa K786031 (→Introduction: New Safety Approach)
- 15:04, 28 September 2012 (diff | hist) . . (-34) . . Part:BBa K786031 (→II.Biobrick design)
- 15:03, 28 September 2012 (diff | hist) . . (-2,939) . . Part:BBa K786031
- 14:58, 28 September 2012 (diff | hist) . . (+34) . . Part:BBa K786031:Design (current)
- 14:57, 28 September 2012 (diff | hist) . . (+33) . . Part:BBa K786031:Design (→Biobrick design)
- 14:55, 28 September 2012 (diff | hist) . . (-8) . . Part:BBa K786031 (→II.Biobrick design)
- 14:55, 28 September 2012 (diff | hist) . . (+1,080) . . Part:BBa K786031
- 14:52, 28 September 2012 (diff | hist) . . (-9) . . Part:BBa K786031:Experience (→Expected Result)
- 14:52, 28 September 2012 (diff | hist) . . (+1) . . Part:BBa K786031:Experience (→Impact & Future application)
- 14:51, 28 September 2012 (diff | hist) . . (+21) . . Part:BBa K786031:Experience (→Impact)
- 14:51, 28 September 2012 (diff | hist) . . (+2) . . Part:BBa K786031:Design
- 14:50, 28 September 2012 (diff | hist) . . (+19) . . Part:BBa K786031:Design
- 14:49, 28 September 2012 (diff | hist) . . (+6) . . Part:BBa K786031:Design
- 14:48, 28 September 2012 (diff | hist) . . (0) . . Part:BBa K786031
- 14:47, 28 September 2012 (diff | hist) . . (+1,448) . . Part:BBa K786031
- 14:45, 28 September 2012 (diff | hist) . . (+1) . . Part:BBa K786031:Experience
- 14:44, 28 September 2012 (diff | hist) . . (-4) . . Part:BBa K786031:Experience
- 14:43, 28 September 2012 (diff | hist) . . (+2,391) . . Part:BBa K786031:Experience
- 14:43, 28 September 2012 (diff | hist) . . (+888) . . N File:Experience3.jpg (Cleavage of selected biobrick Our proposed system can be utilized as a new safety approach for synthetic biology, especially for iGEMers. As we know, one of the most concerned safety issues in engineering cells is that the leakage of bacteria with transf) (current)
- 14:40, 28 September 2012 (diff | hist) . . (+427) . . N File:Experience2.png (To evaluate whether the RSR system transformed into E. coli can degrade genomic DNA and eventually lead to cell death, we measured OD600 of cell cultures every 0.5 h and plotted the growth curves of transformed E. coli (indicated as solid lines) with or w) (current)
- 14:39, 28 September 2012 (diff | hist) . . (+501) . . N File:Experience1.png (We need to test if the proposed system present in the E. coli. We Used primers to amplify the Cas3 and cascade proteins from E. coli K12 genome DNA. Expected band size of cas3 2.7kbp and cascade 4.4kbp are shown in the gel photo, and sequencing has be don) (current)
- 14:37, 28 September 2012 (diff | hist) . . (+1,040) . . Part:BBa K786031:Design (→References)
- 14:37, 28 September 2012 (diff | hist) . . (-1,040) . . Part:BBa K786031:Design
- 14:36, 28 September 2012 (diff | hist) . . (+7) . . Part:BBa K786031:Design
- 14:36, 28 September 2012 (diff | hist) . . (+1,041) . . Part:BBa K786031:Design
- 14:35, 28 September 2012 (diff | hist) . . (+1) . . Part:BBa K786031:Design
- 14:34, 28 September 2012 (diff | hist) . . (+1,917) . . Part:BBa K786031:Design
- 14:33, 28 September 2012 (diff | hist) . . (+297) . . N File:T7rsr graph 4.jpg (A palindrome can be observed by studying some samples of repeat sequences of CRISPR system of E. coli. The secondary structure of pre-crRNA shows that the base pairing helps to form a loop, which may contribute to the Cas3 and Cascade in expression and in) (current)
- 14:32, 28 September 2012 (diff | hist) . . (+757) . . File:T7rsr graph 3.png (current)
- 14:31, 28 September 2012 (diff | hist) . . (0) . . N File:T7rsr graph 3.png
- 14:27, 28 September 2012 (diff | hist) . . (+28) . . Part:BBa K786031
- 14:25, 28 September 2012 (diff | hist) . . (+49) . . N File:T7rsr graph 2.png (a graph desplaying the components of BBa_K786031.) (current)
- 14:23, 28 September 2012 (diff | hist) . . (+27) . . Part:BBa K786031
- 14:22, 28 September 2012 (diff | hist) . . (+54) . . N File:T7rsr graph 1.jpg (a graph showing how t7rsr biobrick (BBa_K786031)works.) (current)
- 08:01, 28 September 2012 (diff | hist) . . (+4,284) . . Part:BBa K786031
- 07:27, 28 September 2012 (diff | hist) . . (-158) . . Part:BBa K786031:Experience (→Applications of BBa_K786031)
- 07:26, 28 September 2012 (diff | hist) . . (+1,559) . . Part:BBa K786031:Experience (→Applications of BBa_K786031)
- 17:13, 27 September 2012 (diff | hist) . . (+1,130) . . Part:BBa K786031:Design (→Design Notes)
