Plasmid_Backbone

Part:BBa_K777133

Designed by: Team Goettingen   Group: iGEM12_Goettingen   (2012-09-28)
Revision as of 14:38, 28 September 2012 by NikoGoe (Talk | contribs)

Similar to pSB1C3, with RFP reporter

This part is similar to J61002 but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2917
    Illegal XbaI site found at 2932
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2917
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
    Illegal NotI site found at 877
    Illegal NotI site found at 2923
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2917
    Illegal XhoI site found at 1901
    Illegal XhoI site found at 2793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2917
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2932
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
    Illegal AgeI site found at 580
    Illegal AgeI site found at 692
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.



Fig.1: Plasmid map of K777133


[edit]
Categories
//function/reporter/fluorescence
//plasmid/construction
//plasmidbackbone/libraryscreening/promoter
Parameters
chassis
copies
resistanceC