Translational_Unit

Part:BBa_K772001:Design

Designed by: Mustafa Elitok   Group: iGEM12_Fatih-Medical   (2012-09-24)
Revision as of 14:27, 28 September 2012 by Mustafa (Talk | contribs) (→‎Design Notes)

EpCAM Binding Dock: C-terminus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
    Illegal NheI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 441


Design Notes

There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.

Also, in order to use this part and to allow this part to work properly, the designer needs to assemble this part with its conjugate: [BBa_K772002][1]

Source

CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.

References