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Part:BBa_K771004

Designed by: Guo Huaqing;Suo Yang   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-25)
Revision as of 06:49, 28 September 2012 by AleAlejandro (Talk | contribs)

Membrane Anchor 4 : ssDsbA-PDZ Domain-LGT-GBD Domain

Membrane anchor 4, which consists of interacting domain and membrane protein Lgt

Membrane anchor 4, which consists of interacting PDZ domain and membrane protein Lgt. Membrane Anchor 4 is proved to constitutively dimerize with Membrane Anchor 3 and 5 [BBa_K771002].

Membrane Accelerator System

Membrane accelerator.jpg


All four membrane proteins are assembled together through interacting domains and ligands. Downstream enzymes are thus assembled.

It has been proved to work as expected by recruiting enzymes involved in Fatty Acid biosynthetic pathway.

Membrane Rudder System

12SJTU-MA1-MA6.jpg

Demonstration of the whole Membrane Rudder device.


Related Biobrick

<br\>Membrabe Anchor 1([BBa_K771001]) <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
    Illegal AgeI site found at 1174
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 345
    Illegal BsaI site found at 795


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Categories
Parameters
n/aMembrane Anchor 4 : ssDsbA-PDZ Domain-LGT-GBD Domain