Coding
Part:BBa_K771101
Designed by: GUO Huaqing;SUO Yang;WU Yuqi Group: iGEM12_SJTU-BioX-Shanghai (2012-09-23)
ssDsbA-Bla
signal recognition particle (SRP)-dependent signaling sequence added before β-Lactamase ,which makes hosts become resistant to Ampicillin
Usage and Biology
This part is basically composed of two parts, ssdsbA, and β-Lactamase. The lactamase DNA was PCR cloned from the vector pETDuet-1, which contains Amplicilin Resistance. the ssdsbA was directly added to the front of lactamase using PCR primer. the ssdsbA can be recognized by signal recognition particle, and the whole protein could be transferred out to the periplasm, endowing the bacteria resistance to Amplicillin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 794
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Categories
Parameters
n/a | ssDsbA-Bla |