Device

Part:BBa_K771206

Designed by: Suo Yang;Guo Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-24)
Revision as of 05:43, 28 September 2012 by Zhiantinglan (Talk | contribs)

Vio D with Membrane Anchor 6

This part is composed with two parts, the Membrane Anchor 6 and Violacein synthetic pathway enzyme vioD. The enzyme can be aggregated with other membrane anchors, composing the Membrane Magic system. For more information, please visit [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai wiki]

Violacein biosynthetic pathway

Violacein is a violet pigment produced by several bacteria through pathway of 5 related genes, vioA, vioB, vioC, vioD and vioE. VioD is one of the key enzyme that function in the production of our main end-product, violacein. The detailed synthetic flux is shown below.

12SJTU VioPathway.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1465
    Illegal XbaI site found at 1477
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1465
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1465
    Illegal XbaI site found at 1477
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1465
    Illegal XbaI site found at 1477
    Illegal AgeI site found at 494
    Illegal AgeI site found at 1339
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 960
    Illegal SapI.rc site found at 2458
    Illegal SapI.rc site found at 2533


[edit]
Categories
Parameters
n/aVio D with Membrane Anchor 6