Reporter
Part:BBa_K900000:Design
Designed by: Austin Jones Group: iGEM12_Berkeley (2012-09-27)
Revision as of 23:31, 27 September 2012 by Austin.jones (Talk | contribs)
PAmCherry (photoactivatable red fluorescent protein)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 682
- 1000COMPATIBLE WITH RFC[1000]
PAmCHERRY is a photoactivatable red fluorescent protein. PAmCherry fluoresces when exposed to 350–400 nm light while does not fluoresce at other wavelengths. It is used a reporter tag; by expressing in cells or localizing to sub-cellular locations, one can gain spatial information within the cell by track organelles, proteins, and the whole cell itself. One can visualize the photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins.
Design Notes
Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.
Source
Synthesized in house. Non-genomic DNA sequence. Mutated version of RFP.