Regulatory

Part:BBa_J100099:Experience

Designed by: Phoebe Parrish   Group: Campbell M Lab   (2012-09-13)
Revision as of 19:55, 27 September 2012 by Phparrish (Talk | contribs) (→‎Applications of BBa_J100099)

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Applications of BBa_J100099

We pipetted 200 microliters of one solution containing E coli cells from a small colony and the activators, one with cells from a small colony and no activators, one containing cells from a large colony and the activators, and one containing cells from a large colony and no activators. We also did a positive control with E coli cells containing a known promoter that causes red florescence (J04450) and a negative control with cells containing a the transcriptional terminator that does not cause red fluorescence (J100091). We tested both fluorescence of our samples using a fluorometer and the light absorbance using a spectrophotometer. We measured the fluorescence and absorbance of five samples of each solution, including a control solution that just contained the growth medium. We averaged the values for each solution and subtracted the average fluorescence/absorbance of the control. We then divided the average fluorescence by the average absorbance for each solution. These values are displayed on the accompanying graph.

Graphs1.png

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