Generator

Part:BBa_K778008

Designed by: Yuka Yamazaki   Group: iGEM12_UT-Tokyo   (2012-09-23)
Revision as of 15:35, 27 September 2012 by YZaki (Talk | contribs) (Safety)

FhlA-E363G generator (pLac-RBS-fhlA363-d.term)

plac-rbs-fhlA363-d.term

FhlA-E363G generator (pLac)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).

This biobrick part can be used to overexpress FhlA-E363G with IPTG induction for enhanced hydrogen production.

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System]



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1457
  • 1000
    COMPATIBLE WITH RFC[1000]


Safety.png

[edit]
Categories
Parameters
None