Coding

Part:BBa_K896010:Design

Designed by: Shih-Hao Chen   Group: iGEM12_NYMU-Taipei   (2012-09-22)
Revision as of 10:58, 27 September 2012 by Lawrence19993 (Talk | contribs) (→‎References)

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smtA ( Metallothionein, Cd2+ binding protein)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 93
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 93
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 93
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SmtA design.jpg

Source

Synechococcus elongatus PCC7942

References

  • Sigel, A.; Sigel, H.; Sigel, R.K.O., ed. (2009). Metallothioneins and Related Chelators. Metal Ions in Life Sciences. 5. Cambridge: RSC Publishing.
  • Jianguo Shi, William P. Lindsay, James W. Huckle, Andrew P. Morby and Nigel J. Robinson. Cyanobacterial metallothionein gene expressed in Escherichia coli Metal-binding properties of the expressed protein. FEB.5 11081 Volume 303, number 2,3, 159-163