Part:BBa_K896011

Mnt (transporter, Cd2+ absorbing molecule) Mnt gene is the Mg2+/Cd2+ transporter which belongs to ABC transporters system. The ATP-binding cassette (ABC) transporters form one of the largest known protein families, and are widespread in bacteria, archaea, and eukaryotes. They couple ATP hydrolysis to active transport. The structure of a prokaryotic ABC transporter usually consists of three components; typically two integral membrane proteins each having six transmembrane segments, two peripheral proteins that bind and hydrolyze ATP, and a periplasmic (or lipoprotein) substrate-binding protein. Many of the genes for the three components form operons as in fact observed in many bacterial and archaeal genomes.

• ZHIQI HAO,1 SHAOLIN CHEN,1 AND DAVID B. WILSON1,2, Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum, APPLIED AND ENVIRONMENTAL MICROBIOLOGY,Nov. 1999, p. 4746–4752 Vol. 65, No. 11

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1478
  Illegal SpeI site found at 1972
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1972
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2519
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1478
  Illegal SpeI site found at 1972
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1478
  Illegal SpeI site found at 1972
  Illegal AgeI site found at 67
  
• 1000
  COMPATIBLE WITH RFC[1000]