Generator

Part:BBa_K797015:Design

Designed by: Tomohiro Nobeyama   Group: iGEM12_Kyoto   (2012-09-22)
Revision as of 03:26, 27 September 2012 by Tomohiro.C.N (Talk | contribs) (→‎References)

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FT generator with Histag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 84


Design Notes

6-Histag is inserted by PCR.


Source

FT gene is derived from Arabidopsis.

References

[1]Paula Teper-Bamnolker and Alon Samach1 (2005) "The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves