DNA
pSC101ts

Part:BBa_K864051

Designed by: Erik Lundin   Group: iGEM12_Uppsala_University   (2012-09-26)
Revision as of 00:56, 27 September 2012 by Erlu2699 (Talk | contribs)

pSC101ts temperature sensitive low copy origin of replication

BBa_K864050 is a pSC101 origin of replication and is found in the pSB8*15 series of BioBrick vectors. This ori is an improvement of the ori found in all pSB4*5 plasmids, BBa_I50042. BBa_I50042 has been shown to have a higher copy number than expected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2130
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid/component/origin
Parameters
None