Part:BBa_K953000
Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)
Synechocystis Heme Oxygenase with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953004 in that it also includes a T7 promoter (Part BBa_I712074). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome(Part BBa_K953001 or Part BBa_K953002). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953004) can be coupled with this part to activate the oxidation of heme to produce biliverdin.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 314
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| n/a | Heme Oxygenase with T7 & RBS (E. coli Codon Optimised) |