Not Released
Sample In stock
Experience: Works
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Reporter
Citrine

Part:BBa_K812030

Designed by: Hafez El Sayyed   Group: iGEM12_Evry   (2012-09-20)
Revision as of 23:55, 26 September 2012 by TriCer (Talk | contribs)

mCitrine reporter with a Kozak sequence for eukaryotic expression

mCitrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This part is in pSB1C3.


This part was characterized in pCS2+ BBa_K812000 which contains the CMV promoter.
J04450 was replaced by this part, it built the BioBrick BBa_K812130. This plasmid (BBa_K812133) express mCitrine into Xenopus:
Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles. perdu

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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