Translational_Unit
AnIn

Part:BBa_K772007:Design

Designed by: Mustafa Elitok   Group: iGEM12_Fatih-Medical   (2012-09-26)
Revision as of 23:54, 26 September 2012 by Mustafa (Talk | contribs) (→‎Design Notes)

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GFP - LasR anchor-inducer protein complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1155
    Illegal AgeI site found at 1352
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 668


Design Notes

Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.

Source

Cleavage site, LasR and GFP sequences are gathered from Registry.

References