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Part:BBa_K812031

Designed by: Chauris Karine   Group: iGEM12_Evry   (2012-09-20)
Revision as of 23:45, 26 September 2012 by TriCer (Talk | contribs)

sfGFP with kozak sequence for eukaryotic expression

Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (BBa_K812000) and related parts. This part is in pSB1C3.


This part was characterized in pCS2+ with CMV promoter BBa_K812000.
J04450 was replaced by this part, it built the BioBrick BBa_K812133. This plasmid (BBa_K812133) express sfGFP into Xenopus:
perdu

The expression of sfGFP is present only in one of the two embryo/tadpole. sfGFP is present only in few tissue as others reporters. sfGFP is expressed in tail's muscles and branchial basket for this tadpole. After injection of this plasmid into Xenopus it does not diffuse in the eggs, and the plasmid express sfGFP in few different tissues.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19


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