Part:BBa_K812013
GFP-AID OsTirI polysistronic system for auxin detection in tadpole
This part is the combination of the BBa_K812010(GFP-AID) and BBa_K812012 (OsTirI) for co-expression in frogs. It is impossible to create polysistronic mRNA in tadpole. In order to overcome the problem, we fused the proteins with a self-cleavable peptide, Pep2A. The protein is transcribed as a single protein and the the peptide cleave itself or with the help of an endogeneous protease giving the two protein, colocalized in the same cell.
This system is a modified version of a pattented device by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
This part can be micro-injected using pSC2+ ( BBa_K812000) or related plasmid as a backbone.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1990
Illegal NotI site found at 1844 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3205
Illegal XhoI site found at 1913 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1612
Illegal NgoMIV site found at 1721
Illegal AgeI site found at 1021 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1406
Illegal BsaI site found at 1636
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