Part:BBa_K812110

GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid

This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. his part is in pCS2+, the plasmid backbone is BBa_K812000 and the device is from BBa_K812010.

This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.

The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.


The tadpole 1 express GFP-aid in epidermic cells, whereas the tadpole 2 express GFP-aid in optical nerve,nostril nerve, tail muscle cells and branchial basket.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1020
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1405