Part:BBa_K864440

Small RNA spot42

The natural small RNA spot42 taken from the E-coli MG1655. Small RNA is belived to interact in many metabolic pathways. The spot42 scaffold interacts with HFQ-proteins. It is in many cases shown to bind into the rbs area of the mRNA and thereby blocking translation initiation. ( V.Sharma, A.Yamamura Y.Yokobayashi, 2011 )

We wanted to use this sRNA for gene downregulation on translational level, in this case block the ribosome from binding to the rbs area.

You can use this natural scaffold to make your own sRNA, inhibiting translation on a gene of your choice. The sRNA is coupled to a strong promotor, J23101

Reference

Engineering Artificial Small RNAs for Conditional Gene Silencing in Escherichia coli Vandana Sharma, Asami Yamamura, and Yohei Yokobayashi ACS Synthetic Biology 2012 1 (1), 6-13

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]