Part:BBa_K801003:Design

pTUM103 yeast shuttle vector with pADH1 promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 36
  Illegal suffix found at 776
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 36
  Illegal NheI site found at 4041
  Illegal SpeI site found at 777
  Illegal PstI site found at 791
  Illegal NotI site found at 42
  Illegal NotI site found at 784
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 36
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 36
  Illegal suffix found at 777
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 36
  Plasmid lacks a suffix.
  Illegal XbaI site found at 51
  Illegal SpeI site found at 777
  Illegal PstI site found at 791
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 149
  Illegal BsaI.rc site found at 2198
  Illegal BsaI.rc site found at 3144
  Illegal SapI site found at 1115
  Illegal SapI site found at 3296
  

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible .

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

Keywords:

Abbreviations:

Design Notes

Related BioBrick:

• BBa_K801002
• BBa_K801001

Quality control measures:
Test digestion using SpeI+PstI-HF

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
pYES2, Invitrogen

Organism:
Designed for the following Chassis: Saccharomyces cerevisiae, Escherichia coli

References

Literature references:

Database references: