Generator
D0

Part:BBa_K738000:Design

Designed by: Huachun Liu   Group: iGEM12_ZJU-China   (2012-07-13)
Revision as of 21:55, 26 September 2012 by Smile (Talk | contribs)

RNA Scaffold generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (rne131) gene to limit RNA degradation.

Safety Notes

The gene products of some biobricks are RNAs and they will not be translated into proteins. As we know, RNA can be easily degraded, so our biobricks will not raise potentially safety problems.

Source

Dr. Camille J. Delebecque offer us d0 secquence in plsmid backbone pETDuet-1(Novagan).

References

C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” Science 333, 470-474 (2011).