Part:BBa_K733004

RBS+ydcE

ydcE which is also named as ndoA, encoding an RNase, namely EndoA. Being in the same RNase family with MazF/ChpAK/PemK, YdcE - the endoribonuclease ydcE encodes - can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC). Thus, overexpression of EndoA can lead to programmed cell death or unhealthy conditions, depending on the amount of EndoA. Note that ydcE(ndoA) is demonstrated functional both in E. coli and B. subtilis. (Pellegrini et al., 2005)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Pellegrini O, Mathy N, Gogos A, Shapiro L, and Condon C. "The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.." Molecular microbiology. 56.5 (2005): 1139-1148. Print.