Composite
CBDclos(T7
Part:BBa_K863112:Design
Designed by: Moritz Müller Group: iGEM12_Bielefeld-Germany (2012-09-20)
Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 329
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added the regulatory unit BBa_K525998 (T7+RBS) in front of the CBD and a short GS-Linker behind the domain and used Freiburg-Standard to be able to fuse proteins to the domain.
Primers that were used to make this BioBrick:
CBDclos_T7RBS | CCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAATAATGTCAGTTGAATTTTACAACTCTAAC |
CBDclos_2ASlink_compl | CTGCAGCGGCCGCTACTAGTATTAACCGGTGCTGCCTGCAAATCCAAATTCAACATATGTATC |
Source
Cloning-vector