Composite
CBDclos(T7

Part:BBa_K863112:Design

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-20)
Revision as of 19:38, 26 September 2012 by Mo (Talk | contribs) (→‎Design Notes)

Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added the regulatory unit BBa_K525998 (T7+RBS) in front of the CBD and a short GS-Linker behind the domain and used Freiburg-Standard to be able to fuse proteins to the domain.

CBDclos_T7RBS CCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAATAATGTCAGTTGAATTTTACAACTCTAAC
CBDclos_2ASlink_compl CTGCAGCGGCCGCTACTAGTATTAACCGGTGCTGCCTGCAAATCCAAATTCAACATATGTATC

Source

Cloning-vector

References