Part:BBa_K801002:Design

pTUM102 yeast shuttle vector with pTEF2 promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 36
  Illegal suffix found at 705
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 36
  Illegal NheI site found at 3970
  Illegal SpeI site found at 706
  Illegal PstI site found at 720
  Illegal NotI site found at 42
  Illegal NotI site found at 713
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 36
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 36
  Illegal suffix found at 706
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 36
  Plasmid lacks a suffix.
  Illegal XbaI site found at 51
  Illegal SpeI site found at 706
  Illegal PstI site found at 720
  Illegal NgoMIV site found at 194
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2127
  Illegal BsaI.rc site found at 3073
  Illegal SapI site found at 1044
  Illegal SapI site found at 3225
  

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible.

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

Keywords:

• pTUM, pYES2, TEF2-Promoter

Abbreviations:

• TEF2

Design Notes

Related BioBrick: pTUM100, pTUM101, pTUM103, pTUM104

Quality control measures:
Testdigestion using XbaI+PstI-HF, and EcorI-HF+SpeI-HF

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity: none

Cytotoxicity:

Safety notes:
health_risk

Intellectual property:

Corresponding part author/authors:

Source

Source:
pYES2, pYES2 Vector kit, company: Invitrogen

Organism:
Designed for E.coli, Yeast

References

Literature references:

Database references: