Composite

Part:BBa_K784039:Design

Designed by: Rachel Lev   Group: iGEM12_Technion   (2012-09-25)
Revision as of 19:19, 26 September 2012 by Sl.ilya (Talk | contribs) (→‎Source)

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T7*(K1F) RNA polymerase + terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We planed to add T7*(K1F) RNAP primers ,that included tails with restriction site, by PCR : XbaI at the 5' end. There wasn’t any necessity to add the suffix restriction sites at the 3' because it was already at the end of the RNAP.


Source

The terminator is from the parts registry (Part:BBa_B0015). RNAP are from: Temme K., et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.

References