Part:BBa_K873003

conjugative transfer of RFG

This part contains a reporter gene I13521,combined with OriTR(the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.)

Function

Once the conjugation take place,the recipient bacterium will obtain the reporter gene which gives
us a signal to distinguish whether the conjugation has happened as well as the conjugation efficiency
easily through observation. In addition, it facilitates the Follow-up experiments on the conjugation
rate, conjugation time, and conjugation efficiency between different strains. 

Results

The TEM image of conjugation:

The agar Luria-Bertani medium of DH5α: The agar Luria-Bertani medium of BL21:

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 117
  Illegal NgoMIV site found at 127
  Illegal AgeI site found at 1014
  Illegal AgeI site found at 1126
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 19