Part:BBa_K819005

Constitutive LuxBrick Generator

Summary

Design

Characterization

Reference

Summary

This part serves as an ultra sensitive photoreceptor (which can sense light as weak as moonlight) and will induce a light-dependent repression of genes under specific promoters like RecA408 promoter and SulA408 promoter.

It is a fusion protein consisting of the DNA binding domain of E.coli endogenous SOS system repressor LexA, and fungus N.crass light sensing protein VVD which forms a rapidly exchanging dimer when excited by blue light. Both the DNA binding doamin and dimerization domain of this protein were mutated at specific sites for better bio-orthogonality and higher on-off ratio. (to see the design page for details, please click here)

We characterized this luminesensor in different ways and all the results showed that this luminesensor worked quite well. (to see the characterization page for details, please click here)

To learn more details about this luminesensor and the related project by Peking 2012 iGEM team, please visit our [http://2012.igem.org/Team:Peking wiki]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3014
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2012
  Illegal XhoI site found at 2842
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4401
  Illegal BsaI.rc site found at 1410
  Illegal SapI.rc site found at 4726